Exopolysaccharide Production From Nostoc sp. Under Different Nutritional Conditions




In the last decades, biotechnological applications have led to the optimization of the cultural conditions for the production of exopolysaccharides (EPSs) by cyanobacteria. Nostoc is a genus of cyanobacteria found in various environments that forms colonies composed of filaments of moniliform cells in a gelatinous sheath. The effect of some important nutritional and growth beside iron nanoparticles on production by Nostoc sp. was studied. Optimization of the cultural conditions was carried out using Central Composite Design (CCD), and it was achieved at pH 8.39, 1% of NaCl and 0.8 g.l-1 nitrate based in modified BG0 medium and more than 12 g.l-1 of EPS was obtained by phenol-sulphuric acid method. The present research investigated the possibility of inducing EPS production in the presence of Fe3O4 nanoparticles from optimized microalga cultures. This study revealed that Nostoc sp. treatment with 10 mg.l-1 Fe3O4 NPs induced maximal EPS production (4 µg.ml-1).


Akin H, Brandam C, Meyer X. M, Strehaiano P. (2008). A model for pH determination during alcoholic fermentation of a grape must by Saccharomyces cerevisiae. Chemistry Engineering Process: Process Intensification. 47: 1986-1993.
Arad S.M, Lerental YB, Dubinsky O. (1992). Effect of nitrate and sulfate starvation on polysaccharide formation in Rhodella reticulata. Biores. Technol. 42: 141-148.
Baky AE, Hana H, Gamal S. El-Baroty, Bouaid A, Martinez M, Aracil J. (2012). Enhancement of lipid accumulation in Scenedesmus obliquus by Optimizing CO2 and Fe3+ levels for biodiesel production. Bioresource Technology. 119: 429-432.
Chengcheng L, Le Z, Hang Y, Roujing-LPT, Xu L, Yaqin Z, Zhan C-, Fengming L. (2017). Self-assembled Exopolysaccharide nanoparticles for bioremediation and green synthesis of noble metal nanoparticles. American Chemical Society. 22808-22818.
Chen W, Chen S, Kumarkhanal S, Sung S. (2006). Kinetic study of biological hydrogen production by anaerobic fermentation. International Journal of Hydrogen Energy. 31: 2170-2178.
Chuandong S. Zhenming C, Weidong L. (2007). Optimization of medium and cultivation conditions for enhanced exopolysaccharide yield by marine Cyanothece sp. 113. Chinese Journal of Oceanology and Limnology. 25 (4): 411-417.
Chi Z and Zhao S. (2003). Optimization of medium and cultivation conditions for pullulan production by a new pullulan-producing yeast. Enzyme and Microb Technology. 32: 206-211.
Dante S, Videla P, Susana GF. (2016). Extracellular Polymeric Substance (EPS). Production by Nostoc minutum -under Different Laboratory Conditions. Advances in Microbiology. 6:374-380.
Delattre C, Pierre G, Laroche C, Michaud P. (2016). Production, extraction and characterization of microalgal and cyanobacterial exopolysaccharides. 34 (7): 1159-1179.
De Philippis R and Vincenzini M. (1998). Exocellular polysaccharides from cyanobacteria and their possible applications. FEMS Microbiology Reviews. 22: 151-175.
Dhanesh K, Petr K, Siba PA. (2018). Exopolysaccharides from cyanobacteria and microalgae and their commercial application. Current Science. 115 (2): 234-241.
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry. 28: 350-356.
Federico R and Roberto DP. (2015). Role of cyanobacterial exopolysaccharides in phototrophic biofilms and in complex microbial mats. Life. 5 (2): 1218-1238.
Freitas F, Cristiana AV, Torres C. AV, Reis MAM. (2017). Engineering aspects of microbial exopolysaccharide production. 245 (Pt B):1674-1683. doi: 10.1016/j.biortech.2017.05.092.
Jttawuttipoka T, Planchon M, Spalla O, Benzerara K, Guyot F, Cassier-Chauvat C, Chauvat F. (2013). Multidisciplinary evidences that Synechocystis PCC6803 exopolysaccharides operate in cell sedimentation and protection against salt and meta stress. Plos One. 8.2: e 55564.
Khosravi Rineh M, Noori M, Ahmadi A. (2011). The study of environmental factors effects on wastewater phytoplankton in Arak. Journal of Plant Environmental Physiology. 5 (4): 1-9.
Krystian M, Waldemar I, Claire R, Aurore R, Dorothee G. (2015). Effect of metals, metalloids and metallic nanoparticles on microalgae growth and industrial production biosynthesis. International Journal of Molecular Science. 16: 23929-23969.
Kumar AS, Mody K, Jha B. (2007). Bacterial exopolysaccharides: a perception. Journal of Basic Microbiology. 47: 103-117.
Lanzhou C, Lina Z, Yongding L, Songqiang D, Hao W, Gaohong W. (2012).Toxicological effects of nanometer Titanium dioxide (nano-TiO2) on Chlamydomonas reinhardtii. Ecotoxicolog and Environmental Safety. 84: 155-162.
Leganes F, Sanchez E, and Fernandez V. (1987). Effect of indole acetic acid on growth and dinitrogen fixation in cyanobacteria, Plant Cell Physiology. 28: 529-533.
Mahmoudi N, Greg FS, Roberta RF. (2011). Comparison of commercial DNA extraction kits for isolation and purification of bacterial and eukaryotic DNA from PAH-contaminated soils. Canadian Journal of Microbiology. 57: 623-628 .
Marker AFH. (1972). The use of acetone and methanol in the estimation of chlorophyll in the presence of phaeophytin. Freshwater Biology. 2. 361-385.
Namita J, Davinder P, Jasvirinder SK. (2013). Optimization, characterization and ï‌‚ow properties of exopolysaccharides produced by the cyanobacterium Lyngbya stagnina. Journal of Basic Microbiology. 53: 902-912.
Nicolaus B, Panico A, Lama L, Romano I, Manca MC, De Giulio A. (1999). Chemical Composition and Production of Exopolysaccharides from Representative Members of Heterocystous and Non-Heterocystous Cyanobacteria. Phytochemistry. 52: 639-647.
Otero A and Vincenzini M. (2003). Extracellular polysaccharide synthesis by Nostoc strains as affected by N source and light intensity. Journal of Biotechnology. 102: 143-152.
Pereira S.B, Mota R, Vieira P.C, Vieira J, Paula T. (2015). Phylum-wide analysis of genes/ proteins related to the last steps of assembly and export of extracellular polymeric substances (EPS) in cyanobacteria. Scientific Report. 1-16.
Pereira S, Zille A , Micheletti E, Moradas-Ferreira P, De Philippis R, and Tamagnini P. (2009). Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly. FEMS Microbiology Reviews. 33: 917.941.
Pereira S, Zille A, Micheletti E, Moradas-Ferreira P, De Philippis R, Tamagnini P.(2009). Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly. FEMS Microbiology Reviews. 33:917-941.
Qurashi AW and Sabri AN. (2012). Bacterial exopolysaccharide and biofilm formation stimulate chickpea growth and soil aggregation under salt stress. Brazilian Journal of Microbiology. 43 (3): 1183-1191.
Stefaniuk M, Oleszczuk P, Ok YS. (2016). Review on nano zerovalent iron (n ZVI): from synthesis to environmental applications. Chemical Engineering Journal. 287: 618-632.
Yuvakkumar R, Elango V, Venkatachalam R, Kannan N, Prabu P. (2011). Influence of Nano Nutrients on Heterocyst-Forming Cyanobacterium, Anabaena. Journal of Metal-.Organic and Nano-Metal Chemistry. 41: 1234-1239.
Zhao S and Chi Z. (2003). A new pullulan-producing yeast and medium optimization for its exopolysaccharide production. Journal of Ocean University of Qingdao. 2: 53-57.