Gold Bioaccumulation by Filamentous Soil Cyanobacteria isolated from Mouteh Goldmine (Isfahan Province, Iran)


This study was aimed to investigate the possible exploitation of phototrophic microorganisms for the oxidation of Au3+ containing composites as well as recovery and bioaccumulation of Au3+ from Mouteh goldmine soil samples. Mechanisms of accumulation including oxidation, dissolution, reduction, leaching and sorption were investigated. After 3 weeks of cyanobacterial growth, gold bioaccumulation from Mouteh gold mine soil solution were determined. Then biomass digested with acidic solutions and subsequently gold amounts were analyzed with Atomic Absorption Spectroscopy. Soil sample with 2 ppm Au3+containing composites ore from Mouteh goldmine were collected. At the end of experiments, the concentration of gold in biomass was almost 6.5 ppm. Results indicated that two soil cyanobacteria species from Mouteh goldmine could bind gold in gold bio-mining process. Bioaccumulation percent was 32% and 30% for Phormidium tenue Gomont and Osillatoria tenuis C. Agardh ex Gomont respectively. Cyanobacterial gold accumulation were determined 6.5 gr/kg biomass dried weight. 

Bio-accumulation, Bio-mining, cyanobacteria, Phormidium tenue, Osillatoria tenuis, Mouteh Goldmine.


Barnett JP, Millard A, Ksibe AZ, Scanlan DJ, Schmid R, Blindauer CA. (2012). Mining genomes of marine cyanobacteria for elements of zinchome- ostasis. Frontiers in Microbiology. 3: 142.

Campbell lR and Martin MH. (1990). Continuous flow fermentation to purify wastewater by the removal of cadmium. Water Air and Soil Pollution. 37: 394-403.

Castenholz RW. (1978). The biogeography of hot spring algal through enrichment cultures. Internationale Vereinigung für Theoretische und Angewandte Limnologie: Mitteilungen. 21: 296-315.

Cavet JS, Borrelly GPM, Robin- son NJ. (2003). Zn, Cu and Co in cyanobacteria: selective control of metal availability. Federation of European Microbiological Societies Microbiology Reviews. 27: 165-181.

Chakraborty N, Banerjee A, Lahiri S, Panda A, Ghosh AN, Pal R. (2008). Biorecovery of gold using cyanobacteria and an eukaryotic alga with special reference to nanogold formation – a novel phenomenon. Journal of Applied Phycology. 21: 145-152.

Dalton B.P. (2008). Lunar Regolith Biomining Workshop Report. NASA Ames Research Center. 32 pp.

Darnall DW, Greene B, Mlchael T, Henzl J, Michael H, Robert A, Pherson Mc., Sneddon J, Alexander MD. (1986). Selective Recovery of Gold and Other Metal Ions from an Algal Biomass. Environmental Science and Technology. 20: 206-208.

Das N. (2010). Recovery of precious metals through biosorption– a review. Hydrometallurgy. 103: 180-189.

Dehghani A, Ostad-rahimi M, Mojtahedzadeh SH, Gharibi KhK. (2009). Journal of the Southern African Institute of Mining and Metallurgy. 10: 417-421.

De Philippis R, Colica G, Micheletti E. (2011). Exopolysaccharide-producing cyanobacteria in heavy metal removal from water: molecular basis and practical applicability of the biosorption process. Applied Microbiology and Biotechnology 92: 697-708.

Desikachary TV. (1959). Cyanophyta. Indian Council of Agricultural Research, New Delhi, 686 pp.

Dissook S, Anekthanakul A, Kittichotirat W. (2013). Screening of gold biomineralization mechanism in cyanobacteria. Procedia Computer Science. 23: 129-136.

Dyer BD, Krumbein WE, Mossman DJ. (1994). Accumulation of gold in the sheath of Plectonema terebrans (filamentous marine cyanobacteria). Geomicrobiology Journal. 12: 91-98.

Eisler R. (2003). Biorecovery of gold. Indian Journal of Experimental Biology. 41: 967-971.

Fogg GE. (1956). The comparative physiology and biochemistry of the blue-green algae. Bacteriological Reveiews. 20: 148-165.

Colica G, Caparrotta S, Bertini G, De Phillipis R. (2012). Gold biosorption by exopolysaccharide producing cyanobacteria and purple nonsulphur bacteria. Journal of applied microbiology. 113: 1380-1388.

Hokmollahi F, Riahi H, Soltani N, Shariatmadari Z, Hakimi-Meybodi MH. (2015). A taxonomic study of blue-green algae based on morphological, physiological and molecular characterization in Yazd province terrestrial ecosystems (Iran). Rostaniha. 16 (2): 152-163.

Kamennaya NA, MAjo-Franklin C, Trent N, Christer J. (2012). Cyanobacteria as Biocatalysts for Carbonate Mineralization. Minerals. 2: 338-364.

Komarek J and Anagnostidis K. (2005). Cyanoprokaryota 2. Teil/2nd Part: Oscillatoriales. p. 759.

Kuyucak N and Volesky B. (1989). The mechanism of gold biosorption. Biorecovery. 1: 219-235.

Lamaia C, Kruatrachuea M, Pokethitiyooka P, Upathamb ES, Soonthornsarathoola V. (2005). Toxicity and accumulation of lead and cadmium in thefilamentous green alga Cladophorafracta (O.F. Muller ex Vahl) Kutzing: A laboratory study. Science Asia. 31: 121-127.

Lengke MF, Ravel B, Fleet ME, Southam G. (2006). Mechanisms of Gold Bioaccumulation by Filamentous Cyanobacteria from Gold (III)−Chloride Complex. Environmental Science and Technology. 40 (20): 6304-9.

Lin Z, Wu J, Xue R, Yang Y. (2005). Spectroscopic characterization of Au3+ biosorption by waste biomass of Saccharomyces cerevisiae. Spectrochim Acta. 61: 761-765.

Mack C, Wilhelmi B, Duncan JR, Burgess JE. (2007). Biosorption of precious metals. Biotechnology Advances. 25: 264-271.

Mata YN, Torres E, Bla´zquez ML, Ballester A, Gonza´lez F, Mun˜oz JA. (2008). Gold (III) biosorption and bioreduction with the brown alga Fucus vesiculosus. Journal of Hazardous Materials. 166: 612-618.

Nicolaus B, Panico A, Lama L, Romano I, Manca MC, de Giulio A, Gambacorta A. (1999). Chemical composition and production of exopolysaccharides from representative members of heterocystous and non-heterocystous cyanobacteria. Phytochemistry. 52: 639-64.

Niu H and Volesky B. (2000). Gold-cyanide biosorption with L-cycsteine. Journal of Chemical Technology & Biotechnology. 75: 436-442.

Pfennig N. (1969). Rhodopseudomonas acidophila, sp. nov. a new species of the budding purple nonsulfur bacteria. Journal of Bacteriology. 99: 597-602.

Pfennig N. (1974). Rhodopseudomonas globformis, sp. nov. a new species of the Rhodospirillaceae. Archives of Microbiology. 100: 197-206.

Prescott GW. (1962). Algae of western great lakes area. WMC. Brown Company Publishing, Iowa, USA. p. 933.

Raymond J and Blankenship RE. (2004). Biosynthetic pathways, gene replacement and theantiquity of life. Geobiology. 2: 199-203.

Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY. (1979). Generic assignments, strain histories and properties of pure culture of cyanobacteria. Journal of General Microbiology. 111: 1-61.

Robinson MG, Brown LN, Hall BD. (1997). Effect of gold (Ш) on the fouling diatom Amphora coffeaeformis; Uptade, toxicity and intractions with copper. Biofouling. 11 (59). Saito MA, Sigman DM, Morel FMM. (2003). The bioinorganic chemistry of the ancient ocean: the co-evolution of cyanobacterial metal requirements and biogeochemical cycles at the Archean-Proterozoic boundary? Inorganica Chimica Acta. 356: 308-318.

Savvaidis I. (1998). Recovery of gold from thiourea solutions using micro-organisms. Biometals. 11: 145-151.

Seiderer T, Venter A, Van Wyk F, Levanets A, Jordaan A. (2017). Growth of soil algae and cyanobacteria on gold mine tailings material. South African Journal of Science. 113: 11-12.

Singh JS. (2014). Cyanobacteria: a vital bio-agent in eco-restoration of degraded lands and sustainable agriculture. Climate Change and Environmental Sustainability. 2: 133-137.

Sutherland IW. (2001). Exopolysaccharides in biofilms, flocs and related structures. Water Science and Technology. 43: 77-86.

Syed S. (2012). Recovery of gold from secondary sources – a review. Hydrometallurgy. 115: 30-51.

Tsuruta T. (2004). Biosorption and recycling of gold using various micro-organisms. Journal of General and Applied Microbiology. 50: 221-228.

Volesky B and May-Phillips HA. (1995). Biosorption of heavy metals by Saccharomyces cerevisiae. Applied Microbiology and Biotechnology 42: 797-806.

Williams RJP and Da Silva JJ RF. (2003). Evolution was chemically constrained. Journal of Theoretical Biology. 220: 323-343.

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