palette
Analysis of Bioactive Compounds in Some Marine Seaweeds along the Coastline of Bandar Abbas, Iran

Abstract

Abstract

Applications of seaweeds as food ingredients and bioactive compounds are increasing these days. Many of seaweeds have to be evaluated for these purposes. This study investigated the biological activities of organic extracts of four green and a red macroalgae collected from the coast of Bandar Abbas, Iran. After species identification, the bioactivities of seaweeds have been studied by antioxidant assay (Ferric reducing power and total antioxidant capacity), total phenolic and flavonoid contents, antibacterial activity, and brine shrimp cytotoxicity activity on model organism, Artemia salina (Linnaeus, 1758) and Artemia franciscana (Kellogg, 1906) were anaysed. The results revealed the most effective algal extracts by maximum antioxidant capacity were recorded using methanol extracts of Ulva intestinalis Linnaeus 1753, and Ulva clathrata (Roth) C. Agardh 1811. In addition, the highest content of total phenolic content was recorded by green seaweeds. While, the highest flavonoid content was obtained from Gracilariopsis persica Bellorin, Sohrabipour & E.C. Oliveira. Most extracts showed significant antibacterial effects, with disk diffusion method. Among the various extracts, the n-hexane extract was found the highest zone of inhibition against analyzed pathogenic bacteria. U. intestinalis and U. clathrate have a better antimicrobial activity and are a potential source of antimicrobial compounds. Further, we found a best toxic effect to A. salina in n-hexane extract of G. persica, LC50 =125 μg/ml. Generally, the findings can be introduced the green seaweeds as priority species for biological properties and can be subject of isolation of the natural antimicrobials.

Keywords
Antibacterial Activity, Antioxidant, Cytotoxicity, Flavonoid Content, Macroalgae, Phenol Content.

References

Bauer A, Kirby W, Sherris JC, Turck M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology. 45: 493.

Cabrita MT, Vale C, Rauter AP. (2010). Halogenated compounds from marine algae. Marine Drugs. 8: 2301-2317.

Chakraborty K, Joseph D, Praveen NK. (2013). Antioxidant activities and phenolic contents of three red seaweeds (Division: Rhodophyta) harvested from the Gulf of Mannar of Peninsular India. Journal of Food Science and Technology. 52: 1924-1935. doi:10.1007/s13197-013-1189-2.

Chandini SK, Ganesan P, Bhaskar N. (2008). In vitro antioxidant activities of three selected brown seaweeds of India. Food Chemistry. 107: 707-713.

Du FY, Li X, Li XM, Zhu LW, Wang BG. (2017). Indolediketopiperazine alkaloids from Eurotium cristatum EN-220, an endophytic fungus isolated from the marine alga Sargassum thunbergii. Marine Drugs. 15: 24.

El-Din SMM, El-Ahwany AM. (2016). Bioactivity and phytochemical constituents of marine red seaweeds (Jania rubens, Corallina mediterranea and Pterocladia capillacea). Journal of Taibah University for Science. 10: 471-484.

Farasat M, Khavari-Nejad R-A, Nabavi SMB, Namjooyan F. (2014). Antioxidant activity, total phenolics and flavonoid contents of some edible green seaweeds from northern coasts of the Persian Gulf. Iranian Journal of Pharmaceutical Research (IJPR). 13: 163.

Ganesan P, Kumar CS, Bhaskar N. (2008). Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds. Bioresource Technology. 99: 2717-2723.

Hu Y, Chen J, Hu G, Yu J, Zhu X, Lin Y, Chen S, Yuan J. (2015). Statistical research on the bioactivity of new marine natural products discovered during the 28 years from 1985 to 2012. Marine Drugs. 13: 202-221.

Kähkönen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen M. (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry. 47: 3954-3962.

Kladi M, Ntountaniotis D, Zervou M, Vagias C, Ioannou E, Roussis V. (2014). Glandulaurencianols A–C, brominated diterpenes from the red alga, Laurencia glandulifera and the sea hare. Aplysia Punctata Tetrahedron Letters. 55: 2835-2837.

Kokabi M and Yousefzadi M. (2015). Checklist of the marine macroalgae of Iran. Botanica Marina. 58: 307-320.

Kokabi M, Yousefzadi M, Feghhi MA, Keshavarz M. (2013). Antioxidant activity of extracts of selected algae from the Persian Gulf, Iran. Journal of the Persian Gulf. 4: 45-50.

Kuda T, Kunii T, Goto H, Suzuki T, Yano T. (2007). Varieties of antioxidant and antibacterial properties of Ecklonia stolonifera and Ecklonia kurome products harvested and processed in the Noto peninsula, Japan. Food Chemistry. 103: 900-905.

Kumar KS, Ganesan K, Rao PVS. (2008). Antioxidant potential of solvent extracts of Kappaphycus alvarezii (Doty) Doty -An edible seaweed. Food Chemistry. 107: 289-295. Doi:10.1016/j.foodchem.2007.08.016.

Lincoln R, Strupinski K, Walker J. (1996). The use of Artemia nauplii (brine shrimp larvae) to detect toxic compounds from microalgal cultures. International Journal of Pharmacognosy. 34: 384-389.

Meenakshi S, Gnanambigai DM, Mozhi ST, Arumugam M, Balasubramanian T. (2009). Total flavanoid and in vitro antioxidant activity of two seaweeds of Rameshwaram coast. Global Journal of Pharmacology. 3: 59-62.

Meenakshi S, Umayaparvathi S, Arumugam M, Balasubramanian T. (2011). In vitro antioxidant properties and FTIR analysis of two seaweeds of Gulf of Mannar. Asian Pacific Journal of Tropical Biomedicine. 1: S66-S70.

Mosaddegh M, Gharanjik BM, Naghibi F, Esmaeili S, Pirani A, Eslami Tehrani B, Keramatian B, Hassanpour A. (2014). A survey of cytotoxic effects of some marine algae in the Chabahar coast of Oman Sea. Research Journal of Pharmacognosy. 1: 27-31.

Narasimhan MK, Pavithra SK, Krishnan V, Chandrasekaran M. (2013). In vitro analysis of antioxidant, antimicrobial and antiproliferative activity of Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata extracts. Jundishapur Journal of Natural Pharmaceutical Products. 8: 151.

Oyaizu M. (1986). Studies on products of browning reaction. The Japanese Journal of Nutritionand dietetics. 44 (6): 307-15.

Parsaeimehr A, Chen YF. (2013). Algal bioactive diversities against pathogenic microbes. Microbiol Pathogens and Strategies for Combating Them: Science, Technology and Education; Méndez-Vilas, A, Edition. 796-803.

Pirian K, Zarei Jeliani Z, Sohrabipour J, Arman M, Faghihi MM, Yousefzadi M. (2017). Nutritional and Bioactivity Evaluation of Common Seaweed Species from the Persian Gulf. Iranian Journal of Science and Technology, Transactions A: Science. Doi:10.1007/s40995-017-0383-x

Pirian K, Zarei Jeliani Z, Arman M, Sohrabipour J, Yousefzadi M. (2020). Proximate analysis of selected macroalgal species from the Persian Gulf as anutritional resource. Tropical Life Sciences Research. 31: 1-17.

Plaza M, Cifuentes A, Ibáñez E. (2008). In the search of new functional food ingredients from algae. Trends in Food Science and Technology. 19: 31-39.

Prieto P, Pineda M, Aguilar M. 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry. 269: 337-341.

Pushparaj A, Raubbin R, Balasankar T. (2014). An antibacterial activity of the green seaweed Caulerpha sertularioides using five different solvents.International of Journal PharmTech Research. 6: 01-05.

Qi H, Liu X, Ma J, Zhang Q, Li Z. (2010). In vitro antioxidant activity of acetylated derivatives of polysaccharide extracted from Ulva pertusa (Cholorophta). Journal of Medicinal Plants Research. 4: 2445-2451.

Sadati N, Khanavi M, Mahrokh A, Nabavi S, Sohrabipour J, Hadjiakhoondi A. (2011). Comparison of antioxidant activity and total phenolic contents of some Persian Gulf marine algae. Journal of Medicinal Plants. 10: 73-79.

Seenivasan R, Indu H, Archana G, Geetha S. (2010). The antibacterial activity of some marine algae from south east coast of India. Journal of Pharmacy Research. 8: 1907-1912.

Selim SA. (2012). Antimicrobial, antiplasmid and cytotoxicity potentials of marine algae Halimeda opuntia and Sarconema filiforme collected from Red Sea Coast. World Academy of Science, Engineering and Technology. 61: 1154-1159.

Shukla R, Mohan T, Vishalakshi B, Chopra D. (2014). Experimental and theoretical analysis of lp⋯π intermolecular interactions in derivatives of 1, 2, 4-triazoles. Crystal Engineering Communication. 16: 1702-1713.

Singleton V and Rossi JA. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 16: 144-158.

Sohrabipour J and Rabei R. (2008). Rhodophyta of Oman Gulf (South East of Iran). Iranian Journal of Botany. 14: 70-74.

Soleimani S, Pirian K, Zarei Jeliani Z, Arman M, Yousefzadi M. (2018). Bioactivity assessment of selected seaweeds from the Persian Gulf, Iran. Journal of Aquatic Ecology. 7: 25-38.

Valifard M, Mohsenzadeh S, Kholdebarin B. (2017). Salinity effects on phenolic content and antioxidant activity of Salvia macrosiphon. Iranian Journal of Science and Technology, Transactions A: Science. 41: 295-300. Doi:10.1007/s40995-016-0022-y

Vinayak RC, Sabu A, Chatterji A. (2011). Bio-prospecting of a few brown seaweeds for their cytotoxic and antioxidant activities. Evidence-based Complementary and Alternative Medicine. 2011: 1-9.

Wang Z, Zhou H, Han J, He Y, Lu L. (2011). Evaluate insecticidal activity using Artemia salina L. Agrochemicals. 50: 261-263.

Willett WC. (2002). Balancing life-style and genomics research for disease prevention. Science. 296:695-698

Zarei Jeliani Z, Mashjoor S, Soleimani S, Pirian K, Sedaghat F, Yosefzadi M. (2017a). Antioxidant activity and cytotoxicity of organic extracts from tThree species of green macroalgae of Ulvaceae from Persian Gulf. Biotechnology Tarbiat Modares University. 9: 59-67

Zarei Jeliani Z, Yousefzadi M, Sohrabi Pour J, Toiserkani H. (2017b). Growth, phytochemicals, and optimal timing of planting Gracilariopsis persica: An economic red seaweed. Journal of Applied Phycology. 30: 525-533.

Zubia M, Robledo D, Freile-Pelegrin Y. (2007). Antioxidant activities in tropical marine macroalgae from the Yucatan Peninsula, Mexico. Journal of Applied Phycology. 19: 449-458.


Refbacks
  • There are currently no refbacks.