Arsenic Removal from Aqueous Media using Scenedesmus obliquus: the Promoting Impact of Microalgae-Bacteria Consortium
Afsaneh Mohkami, Maziar Habibi-Pirkoohi

The present study was conducted to investigate efficacy of unicellular microalga Scenedesmus obliquus either as solo culture or in combination with the bacterium Shewanella sp in removal of arsenic from aqueous media. For this purpose, pure culture of the microalga was fed with arsenic solution and removal rate was monitored. Moreover, impact of pH, temperature and initial biomass was studied. In the second phase, a consortium of S. obliquus- Shewanella was prepared and used to investigate the effect of bacterium presence in terms of removal enhancement. The results showed that pH 7.0, temperature of 29 oC and initial biomass of 0.8 g/L caused the highest rate of arsenic removal. Comparison of pure culture of S. obliquus and S. obliquus- Shewanella consortium revealed that the latter significantly enhanced removal efficacy.
phycoremediation, Scenedesmus obliquus, Shewanella sp, arsenic


Ansari A, Ravindran B, Gupta S, Nasr M, Rawat I, Bux, F. (2019). Techno-economic estimation of wastewater phycoremediation and environmental benefits using Scenedesmus obliquus microalgae. Journal of environmental management. 240: 293-302.

APHA, AWWA, WEF, Standard Methods for the Examination of Water and Wastewater, 22 ed. American Public Health Association, Washington, D.C. 2012

Brutinel E D, Gralnick A. (2012). Shuttling happens: soluble flavin mediators of extracellular electron transfer in Shewanella. Applied Microbiology and Biotechnology. 93(1): 41-48.

Cai T, Park Y, Li Y. (2013). Nutrient recovery from wastewater streams by microalgae: status and prospects. Renewable and Sustainable Energy Reviews. 19: 360-369.

Chen J, Rosen B. (2016). Organoarsenical biotransformations by Shewanella putrefaciens. Environmental science & technology. 50(15): 7956-7963.

Chibuike G, Obiora S. (2014). Heavy metal polluted soils: effect on plants and bioremediation methods. Applied and Environmental Soil Science, 2014.

Duarte P, Almeida R, Fernandes P, Morais D, Lino M, Gomes C. R, Mucha A P. (2019). Bioremediation of bezafibrate and paroxetine by microorganisms from estuarine sediment and activated sludge of an associated wastewater treatment plant. Science of The Total Environment, 655: 796-806.

Fazal T, Mushtaq A, Rehman F, Khan A U, Rashid N, Farooq Xu, J. (2018). Bioremediation of textile wastewater and successive biodiesel production using microalgae. Renewable and Sustainable Energy Reviews. 82: 3107-3126.

García D, de Godos I, Domínguez C, Turiel S, Bolado S, Muñoz, R. (2019). A systematic comparison of the potential of microalgae-bacteria and purple phototrophic bacteria consortia for the treatment of piggery wastewater. Bioresource technology. 276: 18-27.

Gupta S K, Ansari, F, Shriwastav A, Sahoo N K, Rawat I, Bux, F. (2016). Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for bio-fuels. Journal of cleaner production. 115: 255-264.

Hau H H, Gilbert A, Coursolle D, Gralnick J A. (2008). Mechanism and consequences of anaerobic respiration of cobalt by Shewanella oneidensis strain MR-1. Appl. Environ. Microbiol. 74(22): 6880-6886.

Hülsen T, Hsieh K, Lu Y, Tait S, Batstone D J. (2018). Simultaneous treatment and single cell protein production from agri-industrial wastewaters using purple phototrophic bacteria or microalgae–A comparison. Bioresource technology. 254: 214-223.

Humenik F J, Hanna Jr G P. (1971). Algal-bacterial symbiosis for removal and conservation of wastewater nutrients. Journal (Water Pollution Control Federation). 67: 580-594.

Jafari S A, Cheraghi S, Mirbakhsh M, Mirza R, Maryamabadi A. (2015). Employing response surface methodology for optimization of mercury bioremediation by Vibrio parahaemolyticus PG02 in coastal sediments of Bushehr, Iran. CLEAN–Soil, Air, Water, 43(1): 118-126.

Ji M K, Abou-Shanab R A, Hwang J H, Timmes T C, Kim H C, Oh Y K, Jeon B. H. (2013). Removal of nitrogen and phosphorus from piggery wastewater effluent using the green microalga Scenedesmus obliquus. Journal of Environmental Engineering. 139(9): 1198-1205.

Jia H, Yuan Q. (2016). Removal of nitrogen from wastewater using microalgae and microalgae–bacteria consortia. Cogent Environmental Science. 2(1): 1275089.

Khadem Moghadam Igdelou N, Golchin A. (2019). Risk assessment of contamination of the country's soil and water resources with arsenic. Iranian Journal of Soil and Water Research. 50 (2): 259-272.

Kumar K S, Dahms H U, Won E J, Lee J S, Shin K H. (2015). Microalgae–A promising tool for heavy metal remediation. Ecotoxicology and environmental safety. 113: 329-352.

León R, Cejudo A. G, Fernández, E. (Eds.). (2008). Transgenic microalgae as green cell factories (Vol. 616). Springer Science & Business Media.

Li B, Huang W, Zhang C, Feng S, Zhang Z, Lei Z, Sugiura N. (2015). Effect of TiO2 nanoparticles on aerobic granulation of algal–bacterial symbiosis system and nutrients removal from synthetic wastewater. Bioresource technology. 187: 214-220.

Ma X, Zhou W, Fu Z, Cheng Y, Min M, Liu Y, Ruan R. (2014). Effect of wastewater-borne bacteria on algal growth and nutrients removal in wastewater-based algae cultivation system. Bioresource technology. 167: 8-13.

Martınez M E, Sánchez S, Jimenez J M, El Yousfi F, Munoz L. (2000). Nitrogen and phosphorus removal from urban wastewater by the microalga Scenedesmus obliquus. Bioresource technology. 73(3): 263-272.

McGriff E C, McKinney R. E. (1972). The removal of nutrients and organics by activated algae. Water research. 6(10): 1155-1164.

Megharaj M, Ramakrishnan B, Venkateswarlu K, Sethunathan N, Naidu R. (2011). Bioremediation approaches for organic pollutants: a critical perspective. Environment international. 37(8): 1362-1375.

Monteiro C M, Castro P M, Malcata F X. (2009). Use of the microalga Scenedesmus obliquus to remove cadmium cations from aqueous solutions. World Journal of Microbiology and Biotechnology. 25(9): 1573-1578.

Perales-Vela H V, Pena-Castro J M, Canizares-Villanueva R. O. (2006). Heavy metal detoxification in eukaryotic microalgae. Chemosphere. 64(1): 1-10.

Rasoul-Amini S, Montazeri-Najafabady N, Shaker S, Safari A, Kazemi A, Ghasemi Y. (2014). Removal of nitrogen and phosphorus from wastewater using microalgae free cells in bath culture system. Biocatalysis and Agricultural Biotechnology. 3(2): 126-131.

Sarı A, Uluozlü Ö D, Tüzen M. (2011). Equilibrium, thermodynamic and kinetic investigations on biosorption of arsenic from aqueous solution by algae (Maugeotia genuflexa) biomass. Chemical Engineering Journal. 167(1): 155-161.

Şen A, Pereira H, Olivella M A, Villaescusa I. (2015). Heavy metals removal in aqueous environments using bark as a biosorbent. International Journal of Environmental Science and Technology. 12(1): 391-404.

Sibi G. (2014). Biosorption of arsenic by living and dried biomass of fresh water microalgae-potentials and equilibrium studies. Journal of Bioremediation and Biodegradation. 5(6).

Solimeno A, García J. (2017). Microalgae-bacteria models evolution: From microalgae steady-state to integrated microalgae-bacteria wastewater treatment models–A comparative review. Science of The Total Environment. 607: 1136-1150.

Sulaymon A H, Mohammed A A, Al-Musawi T J. (2013). Competitive biosorption of lead, cadmium, copper, and arsenic ions using algae. Environmental Science and Pollution Research. 20(5): 3011-3023.

Sultana J, Farooqi A, Ali U. (2014). Arsenic concentration variability, health risk assessment, and source identification using multivariate analysis in selected villages of public water system, Lahore, Pakistan. Environmental monitoring and assessment. 186(2): 1241-1251.

Turki Y, Mehri I, Lajnef R, Rejab A B, Khessairi A, Cherif H, Hassen A. (2017). Biofilms in bioremediation and wastewater treatment: characterization of bacterial community structure and diversity during seasons in municipal wastewater treatment process. Environmental Science and Pollution Research. 24(4): 3519-3530.

Vala A K, Sutariya, V. (2012). Trivalent arsenic tolerance and accumulation in two facultative marine fungi. Jundishapur Journal of Microbiology. 5(4): 542-545.

Wang J, Chen, C. (2009). Biosorbents for heavy metals removal and their future. Biotechnology advances, 27(2), 195-226.

Xiong J Q, Kurade M B, Abou-Shanab R A, Ji M K, Choi J, Kim J O, Jeon, B H. (2016). Biodegradation of carbamazepine using freshwater microalgae Chlamydomonas mexicana and Scenedesmus obliquus and the determination of its metabolic fate. Bioresource technology. 205: 183-190.

Zhang B, Lens P N, Shi W, Zhang R, Zhang Z, Guo Cui F. (2018). Enhancement of aerobic granulation and nutrient removal by an algal–bacterial consortium in a lab-scale photobioreactor. Chemical Engineering Journal. 334: 2373-2382.

Zhou G J, Peng F Q, Zhang L, Ying G G. (2012). Biosorption of zinc and copper from aqueous solutions by two freshwater green microalgae Chlorella pyrenoidosa and Scenedesmus obliquus. Environmental Science and Pollution Research. 19(7): 2918-2929.

  • There are currently no refbacks.