Biodiversity of Cultivable Green Algae Collected From the Coast of Guilan

In this study, the cultivable algae in the coasts of Guilan province in the south west part of the Caspian Sea were isolated, purified and then morphologically and molecularly identified in February 2016. The separated samples were examined by optical microscopy and morphological analysis. Then, 18S rDNA gene primer pair was used for green algae polymerase chain reaction. Totally, 14 algae strains were identified. Among them, four strains were related to the algal branch of Chlorophyta. Considering the importance of the Caspian Sea as one of the largest aquatic habitats in the country, many studies have been conducted on the identification and study of phytoplankton and algae of the Caspian Sea. The seasonal variation of phytoplanktons in the southern basin of the Caspian Sea was studied. During a winter season, 31 species belonged to the Chlorophyta among 32 study stations. The results of this research show that the growth and diversity of the algae in the habitats is affected by seasonal fluctuations and changes in environmental factors such as temperature, salinity, food level, type of bed (due to spread range of sampling area) and a combination of them. In general, research has shown that some factors such as salinity, temperature, source of nitrogen and ambient oxygen, pH, heavy elements, UV rays and other environmental stressors affect the chemical composition and antioxidant activity in algae.
Guilan Province, algal isolation, morphology, molecular identification, biodiversity


Afraei M, Nasrollahzadeh H, Roohi A, Makhlough A, Nourbakhsh Kh, Tahami F, RoshanTabari M; Naderi M, Darya-Nabard Gh, Ramezani H, Islami F. (2016). Study of ecological relationships among biological groups of phytoplankton, zooplankton, Jelly comb and macrobenthos at the southeast of the Caspian Sea (Mazandaran-Goharbaran). Iranian Scientific Fisheries Journal. 26 (5): 23-31.

Aladin NB and Plotnikov IS. (2004). The Caspian Sea, Lake Basin Management Initiative. The Caspain Bulletin. 4: 112-126.

Andersen RA. (2005). Algal culturing techniques. Elsevier Academic Press, New York. 578 p.

Berard A, Dorigo U, Humbert J.F, and Martin-Laurent F. (2005). Microalgae community structure analysis based on 18S rDNA amplification from DNA extracted directly from the soil as a potential bioindicator. Agromomy for sustainable development, Springer Veleg, EDP Sciences, INRA. 25 (2): 285-291.

Bock C, Luo W, Kusber W, Hegewald E. (2013). Classification of crucigenoid algae: phylogenetic position of the reinstated genus Lemmermannia, Tetrastrum spp., Crucigenia tetrapedia, and C. lauterbornii (Trebouxiophyceae, Chlorophyta). Journal of Phycology. 49 (2): 329-339..

De Morais MG, da Silva Vaz B, de Morais EG, Vieira Costa JA. (2015). Biologically Active Metabolites Synthesized by Microalgae. BioMed Research International. Article ID: 83576. 15 pp.

Eghtesadi Sh, Zahedi R. (2010). Study of factors affecting South Caspian Sea’s fluctuations of the water level. Research Center of the Atmospheric and Oceanic Sciences of the Iranian Meteorological Organization. 10 (3): 4-13.

El-Gamal AA. (2010). Biological importance of marine algae. Saudi Pharmaceutical Journal. 18 (1): 1–25.

Ferreira P, Soares LA, Costa JA. (2013). Microalgas: umafonte alternative anaobtenção de ácidos gordoses senciais. Revista de Ciências Agrárias. 36: 275-287.

Ganjian Khanari A, Shakouri M, Golichi A, Ghasemnejad M. (2013). The effect of sodium bicarbonate on the growth of algae Scenedesmus sp. in TMRL (AG) medium. Journal of Fisheries. 7 (4): 85-92.

Geitler L. (1932). Cyanophyceae. In: L. Rabenhorst Kryptogamen-Flora. 14. Band. Akademische Verlags gesellschaft, Leipzig, 1196 pp.

Ghahreman A, Naghinejad A, Attar F. (2004). The habitats and flora of the Chamkhaneh-Jirbagh coastal region and the Amirkalayeh coastal wetlands. Journal of Ecology. 33: 46-67.

Haddad R, Alemzadeh E, Ahmadi A, Hosseini R, Moezzi M. (2014). Identification of Chlorophyceae based on 18S rDNA sequences from Persian Gulf. Iranian Journal of Microbiology. 6 (6): 437-442.

Hassan Soltan T, Noroozi M, Amozgar M. (2016). Investigating the rate of chlorophyll a and b and total carotenoids and antioxidant activity of four algae species of the Caspian Sea at Golestan coast. New Journal of Cell-Molecular Biotechnology. 6 (24): 31-36.

Heidari S, Hadian A, MahbobiSofiani N. (2011). Effects of different levels of Nitrate and Ammonium in culture medium for the growth of algae Scenedesmus quadricauda,. Journal of Iranian Fisheries and Natural Resources. 64 (1): 29-39.

Ilavarasi A, Mubarakali D, Praveenkumar R, Baldev E, Thajuddin. (2011). Optimization of Various Growth Media to Freshwater Microalgae for Biomass Production. Biotechnology. 10: 540-545.

John DM, Whitton BA, Brook AJ. (2002). The Freshwater Algal Flora of the British Isles: An Identification Guide to Freshwater and Terrestrial Algae. Cambridge University Press.

Johnson I, Zackary Z, Erik R, Coe Allison M, Nathan PE, Woodward MS. Chisholm SM. (2006). Niche partitioning among Prochlorococcus ecotypes along ocean-scale environmental gradients. Science. 311: 1737-1740.

Kostianoy A and Kosarev A. (2005). The Caspian Sea Environment, (Handbook of Environmental Chemistry). Volume 5, Part P, Springer-Verlag, Germany. Pp. 1-3.

Kulasooriya SA. (2011). Cyanobacteria: Pioneers of Planet Earth. Ceylon Journal of Science. 40 (2): 71-88.

Mahmoudi N, Ahmadi M, Baba-Nejad M, Seifabadi J. (2017). Seasonal distribution of dominant phytoplanktons in the southern Caspian Sea (Mazandaran coastline) and its relationship with environmental factors. Journal of Marine Science and Technology. 16 (1): 87-101.

Nasrollahzadeh H, Makhlough A, Rahmati R, Tahami F, Kayhan Sani A, Gol-Aghaei M. (2015). Study of the stability and turbulence situation in the Caspian Sea ecosystem (Iranian coast) based on the phytoplankton structure pattern. Research Scientific Journal of Ocean Biology, Islamic Azad University. 26: 27-44.

Noroozi M, Amozegar M, Rahimi R, Shahzade Fazeli A. (2017). The isolation and preliminary charachterization of native cyanobacterial and microalgal strains from lagoons contaminated with petroleum oil in Khag Island. Biological Journal of Microorganisms. 5 (20): 33-41.

Olmos J. Paniagua GJ. Contreraf R. 2000. Molecular identification of Dunaliella utilizing the 18r DNA gene. Letters in Appllies Microbiology. 30: 80-84.

Rabiei R, Sohrabi J. (2007). Deep changes in the coverage and biomass percentage of Agarophyte algae of Gracilaria salicornia Dawson (C. Agardh) and its distribution on the Persian Gulf coast. Journal of Research in Iranian Medical Herbs and Aromatic Flowers. 3 (1): 23-38.

Ramezani Aval Riabi H, Azin M, Sheikhi A. (2014). Optimization of Chlorella Vulgaris microalgae medium for increasing starch production in Iran. Journal of Marine Science and Technology. Doi: 10.22113/ jmst. 2016. 33864.

Richmond A. (2008). Handbook of microalgal culture: biotechnology and applied phycology. John Wiley and Sons, New York.

Tahami F, Mezlan A, Negarestan H. (2012). Phytoplankton combination in the Southern part of Caspian Sea. World Apllied Sciences Journal. 16 (1): 99-105.

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