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Gene Expression Analysis in Dunaliella salina Under Salt Stress
salehe Ganjali, فرج اله شهریاری, محمد حسین احمدزاده

Abstract
Different salt concentrations are found in the large portion of water resources and ‎agriculture lands in which most of the plant species cannot be grown. Some microalgae species can be grown either in seawater or in brackish water which is not usable for normal agriculture. The present study analyzed the gene expressions associated ‎with salt stress in Dunaliella Salina. The expression of three coding genes namely ‎acetyl-CoA carboxylase (ACC), channelrhodopsin-1 DChR1 protein (DC), and sodium-coupled phosphate transporter protein (DSSPT) were evaluated at three salinity levels: 3 gL-1 (low), 32 gL-1 (control), 100 gL-1 (high)‎. The analyzes were performed on the 5th, 10th and 17th day of culture. The results revealed that for Low and high salt treatments, the lowest gene expressions were observed on the 5th day of growth, however, the gene ‎expressions were increased with the growth of algae cells until the ‎‎10th day. It should be noted that, under low salinity conditions (3 gL-1), gene ‎expressions were lower than high salinity concentration (100 gL-1) and (32 gL-1) control during the growth.
Keywords
Microalgae, Saltwater, Dunaliella Salina, Gene expression, Salt stress

References

Abdel-Raouf N, Al-Homaidan A, Ibraheem I (2012) Agricultural importance of algae African Journal of Biotechnology 11:11648-11658

Allahverdiyev S, Atilla A, Ismail BS, Sahmurova A (2011) Response of photosystem II and photosynthetic pigments to salt and Baikal EM1 in tree seedlings African Journal of Biotechnology 10:535-538

Araújo O, Gobbi C, Chaloub R, Coelho M (2009) Assessment of the impact of salinity and irradiance on the combined carbon dioxide sequestration and carotenoids production by Dunaliella salina: a mathematical model Biotechnology and bioengineering 102:425-435

Bahieldin A et al. (2014) Control of glycerol biosynthesis under high salt stress in Arabidopsis Functional Plant Biology 41:87-95

Ben-Amotz A (2004) 13 Industrial Production of Microalgal Cell-mass and Secondary Products–Major Industrial Species Handbook of microalgal culture: biotechnology and applied phycology:273

Ben-Amotz A, Avron M (1973) The role of glycerol in the osmotic regulation of the halophilic algae Dunaliella parva Plant physiology 51:875-878

Ben-Amotz A, Avron M (1990) The biotechnology of cultivating the halotolerant alga Dunaliella Trends in biotechnology 8:121-126

Ben-Amotz A, Polle JE, Rao DS (2009) The alga Dunaliella: biodiversity, physiology, genomics and biotechnology. Science Publishers Enfield, NH,

Berliner M (1986) Proteins in Chlorella vulgaris Microbios 46:199-203

Chang SC, Cho MH, Kang BG, Kaufman PB (2001) Changes in starch content in oat (Avena sativa) shoot pulvini during the gravitropic response Journal of experimental botany 52:1029-1040

Cubero B et al. (2009) The phosphate transporter PHT4; 6 is a determinant of salt tolerance that is localized to the Golgi apparatus of Arabidopsis Molecular plant 2:535-552

Daram P, Brunner S, Rausch C, Steiner C, Amrhein N, Bucher M (1999) Pht2; 1 encodes a low-affinity phosphate transporter from Arabidopsis The Plant Cell 11:2153-2166

Deng X, Cai J, Li Y, Fei X (2014) Expression and knockdown of the PEPC1 gene affect carbon flux in the biosynthesis of triacylglycerols by the green alga Chlamydomonas reinhardtii Biotechnology letters 36:2199-2208

Ernst OP, Murcia PAS, Daldrop P, Tsunoda SP, Kateriya S, Hegemann P (2008) Photoactivation of channelrhodopsin Journal of Biological Chemistry 283:1637-1643

Fan J, Cui Y, Wan M, Wang W, Li Y (2014) Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosa under three nutrition stressors Biotechnology for Biofuels 7:1-14 doi:10.1186/1754-6834-7-17

Gimmler H, Weis U, Weis C, Kugel H, Treffny B (1989) Dunaliella acidophila (Kalina) Masyuk—an alga with a positive membrane potential New phytologist 113:175-184

Gomez PI, Barriga A, Cifuentes AS, Gonzalez MA (2003) Effect of salinity on the quantity and quality of carotenoids accumulated by Dunaliella salina (strain CONC-007) and Dunaliella bardawil (strain ATCC 30861) Chlorophyta Biological research 36:185-192

Ho S-H et al. (2017) Dynamic metabolic profiling together with transcription analysis reveals salinity-induced starch-to-lipid biosynthesis in alga Chlamydomonas sp. JSC4 Scientific Reports 7

Holford I (1997) Soil phosphorus: its measurement, and its uptake by plants Soil Research 35:227-240

Katz A, Pick U (2001) Plasma membrane electron transport coupled to Na+ extrusion in the halotolerant alga Dunaliella Biochimica et Biophysica Acta (BBA)-Bioenergetics 1504:423-431

Katz A, Pick U, Avron M (1992) Modulation of Na+/H+ antiporter activity by extreme pH and salt in the halotolerant alga Dunaliella salina Plant physiology 100:1224-1229

Kim M, Park S, Polle JE, Jin E (2010) Gene expression profiling of Dunaliella sp. acclimated to different salinities Phycological Research 58:17-28

Li Q, Gao X, Sun Y, Zhang Q, Song R, Xu Z (2006) Isolation and characterization of a sodium-dependent phosphate transporter gene in Dunaliella viridis Biochemical and biophysical research communications 340:95-104

Liska AJ, Shevchenko A, Pick U, Katz A (2004) Enhanced photosynthesis and redox energy production contribute to salinity tolerance in Dunaliella as revealed by homology-based proteomics Plant physiology 136:2806-2817

Meseck SL, Alix JH, Wikfors GH (2005) Photoperiod and light intensity effects on growth and utilization of nutrients by the aquaculture feed microalga, Tetraselmis chui (PLY429) Aquaculture 246:393-404

Miura K, Sato A, Ohta M, Furukawa J (2011) Increased tolerance to salt stress in the phosphate-accumulating Arabidopsis mutants siz1 and pho2 Planta 234:1191-1199

Nagel G et al. (2003) Channelrhodopsin-2, a directly light-gated cation-selective membrane channel Proceedings of the National Academy of Sciences 100:13940-13945

Patil PD et al. (2011) Optimization of direct conversion of wet algae to biodiesel under supercritical methanol conditions Bioresource technology 102:118-122

Pattison-Granberg J, Persson BL (2000) Regulation of cation-coupled high-affinity phosphate uptake in the yeast Saccharomyces cerevisiae Journal of bacteriology 182:5017-5019

Sahoo PBnAD (2015) Classification of Algae Cellular Origin, Life in Extreme Habitats and Astrobiology

Sayanova O, Mimouni V, Ulmann L, Morant-Manceau A, Pasquet V, Schoefs B, Napier JA (2017) Modulation of lipid biosynthesis by stress in diatoms Philosophical Transactions of the Royal Society B: Biological Sciences 372:20160407

Schneider F, Gradmann D, Hegemann P (2013) Ion selectivity and competition in channelrhodopsins Biophysical journal 105:91-100

Schneider F, Grimm C, Hegemann P (2015) Biophysics of channelrhodopsin Annual review of biophysics 44:167-186

Shariati M, Hadi MR (2011) Microalgal biotechnology and bioenergy in Dunaliella From Analysis and Modeling to Technology

Sineshchekov OA, Govorunova EG, Spudich JL (2009) Photosensory functions of channelrhodopsins in native algal cells Photochemistry and photobiology 85:556-563

Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Commercial applications of microalgae Journal of bioscience and bioengineering 101:87-96

Spudich JL, Sineshchekov OA, Govorunova EG (2014) Mechanism divergence in microbial rhodopsins Biochimica et Biophysica Acta (BBA)-Bioenergetics 1837:546-552

Werman MJ, Mokady S, Ben-Amotz A (2002) Bioavailability of the isomer mixture of phytoene and phytofluene-rich alga Dunaliella bardawil in rat plasma and tissues The Journal of nutritional biochemistry 13:585-591

Zheng H-Q, Chiang-Hsieh Y-F, Chien C-H, Hsu B-KJ, Liu T-L, Chen C-NN, Chang W-C (2014) AlgaePath: comprehensive analysis of metabolic pathways using transcript abundance data from next-generation sequencing in green algae BMC genomics 15:196

Zhu W, Miao Q, Sun D, Yang G, Wu C, Huang J, Zheng C (2012) The mitochondrial phosphate transporters modulate plant responses to salt stress via affecting ATP and gibberellin metabolism in Arabidopsis thaliana PloS one 7:e43530


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