Effects of light intensity and photoperiod on growth rate and biomass production in green algae Scenedesmus dimorphus

Effects of photoperiod, light intensity and nitrate levels on growth (cell number) and biomass of Scenedesmus dimorphus was studied. Three levels of photoperiod 12:12, 8:16 and 16:8 hours dark: light, light intensity of 3000, 5000 and 7000 lux and nitrate levels of 1.47, 2.94 and 4.41 mmol/l were set up. Algae culture was performed at 28±2 °C in BBM culture medium. Algae cells were counted every three days for one month. By the end of experiments the lowest cell concentration (93×105 cell/l) was observed at 12:12 photoperiod, 1.47 mmol/l nitrate and 3000 lux light intensity and the highest cell concentration at 8:16 photoperiod, 2.97 mmol/l nitrate and 3000 lux light intensity. Increase in photoperiod correlated with significant increase in cell concentration. Higher light intensity with lower nitrate levels resulted in higher cell concentrations. The lowest algal biomass (1.38 gr.) was observed at 12:12 photoperiod, 3000 lux light intensity and 1.47 mmol/l nitrate levels while the highest biomass (5.2 gr.) at 8:16 photoperiod, 7000 lux light intensity of and 2.94 mmol/l nitrate level.
Scenedesmus dimorphus, light intensity, photoperiod, growth rate, fatty acids


Balat H. 2010. Prospects of biofuels for a sustainable energy future: a critical assessment. Energy Educ. Sci. Technol. Part A. 24: 85. 111.

Dittamart D, Chayakorn P, Jeeraporn P, Yuwadee P. 2014. Effects of organic carbon source and light-dark period on growth and lipid accumulation of Scenedesmus sp. AARL G022. Maejo Int. J. Sci. Technol. 2014, 8(02), 198-206

Ganjian A. 2010. Workshop on algae culture, Caspian Fisheries pollution research group, 33 pages

George B, Pancha I, Desai C, Chokshi K, Paliwal C, Ghosh T. 2014. Effects of different media composition, light intensity and photoperiod on morphology and physiology of freshwater microalgae Ankistrodesmus falcatus – a potential strain for biofuels production. Bioresource Techno 2014; 171:367–74.

Guedes AC, Meireles LA, Amaro HM, Malcata, FX 2010. Changes in lipid class and fatty acid composition of cultures of Pavlova lutheri, in response to light intensity. Journal of the American Oil Chemists Society 87 (7), 791–801.

Hanna H, EL-Baky Hussein MM, EL-Baroty G. 2008. Algal extracts improve antioxidant defense abilities and salt tolerance of wheat plant immigrated with sea water Electronic, Journal of Environmental, Agricultural and food chemistry, 6, 2812-2832.

Isik O, Hizaric S, Sayin S, Gokpinar Durmaz Y. 2006. The effect of the environmental factors on the vitamin C (ascorbic acid), E (alpha – tocopherol), β-carotene contents and the fatty acid composition of Spirulina platensis. J. Fisheries & Aquatic Sciences. 3(4): 257 – 261.

Kianmehr, H. 2005. Freshwater algae of Mashhad and adjacent regions, Ferdowsi University of Mashhad

Klyachko-Gurvich GL, Tsoglin LN, Doucha J, Kopetskii J, Shebalina IB, Semenenko VE. 1999. Desaturation of Fatty Acids as an Adaptive Response to Shifts in Light Intensity. J. Physiol. Plant. 107: 240–249.

Leon-Banares R, Gonzalez-Ballester D, Galvan A, Fernandez E. 2004. Transgenic microalgae as green cell factories. J. Trends Biotechnol. 22:45-52.

Liang K, Zhang Q, Gu M, Cong W. 2013. Effect of phosphorus on lipid accumulation in freshwater microalga Chlorella sp. J. Appl. Phycol. 25, 311- 318.

Liang Y, Sarkany N, Cui Y. 2009. Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions. J. Biotechnol. Lett. 31: 1043-1049.

Mandotra SK, Kumar P, Suseela MR, Nayaka S, Ramteke PW.2015. Evaluation of fatty acid profile and biodiesel properties of microalga Scenedesmus abundans under the influence of phosphorus, pH and light intensities, Bioresource Technology2015. doi:

Mata TM. 2010. Microalgae for biodiesel production and other applications: areview. J. Renew Sustainable Energy Rev. 14: 217-232.

Pancha I, Chokshi K, Mishra S. 2015. Enhanced biofuel production potential with nutritional stress amelioration through optimization of carbon source and light intensity in Scenedesmus sp. CCNM 1077. Bioresour Technol 2015; 179:565–72.

Pribyl P, Pilny J, Cepak V, Kastanek P. 2016. The role of light and nitrogen in growth and carotenoid accumulation in Scenedesmus sp., Algal Research, 16(2016) 69-75

Rai PM and Gupta Sh. 2016. Effect of media composition and light supply on biomass, lipid content and FAME profile for quality biofuel production from Scenedesmus abundans, Energy Conversion and Management, xxx (2016) xxx–xxx

Rivkin R.B. 1989. Influence of irradiance and spectral quality on the carbon metabolism of phytoplankton. I. Photosynthesis, chemical composition and growth. J. Marine Ecology Progress Series. 5:291- 304.

Rosenberg JN, Oyler GA, Wilkinson L, Betenbaug MJ. 2008. A green light for engineered algae: redirecting metabolism to fuel a biotechnology revolution. Journal of Current Opinion in Biotechnology, 19: 430-436.

Ruangsomboon S. 2012. Effect of light, nutrient, cultivation time and salinity on lipid production of newly isolated strain of the green microalga, Botryococcus braunii KMITL 2. Bioresour. Technol. 109, 261-265.

Scott SA, Davey MP, Dennis JS, Horst I, Howe CJ, Lea-Smith DJ, Smith AG. 2010. Biodiesel from algae: challenges and prospects. Curr. Opin. Biotechnol. 21, 277-286.

Shokravi Sh, Soltani N, baftehChi L. 2002. Compiling methodology of using cyanobacteria as biological manure in rice fields, Research project report, Applied and basic science research Institute, Jahad Daneshgahi, shaheed Behesthi University

Singh SP and Singh P. 2015. Effect of temperature and light on the growth of algae species: A review Renewable and Sustainable Energy Reviews50 (2015)431–444

Soltani N, BaftehChi L, Shokravi Sh. 2002. Survey on industrial and pharmaceutical aspects of algae with stress on species identified from Iran, Research project report, Applied and basic science research Institute, Jahad Daneshgahi, Shaheed Behesthi University.

Soltani N, Khavarinezhad R, Riahi H. 1992. Effects of photoperiod on growth status and pigments of Scenedesmus brevispin, M. Sc. Thesis, tarbiat Moalem University.

Spijkerman, E and Wacker A. 2011. Interactions between P-limitation and different C conditions on the fatty acid composition of an extremophile microalgae. Extremophiles 15, 597-609.

Sun X, Cao Y, Xu H, Liu Y, Sun J, Qiao D, Cao Y. 2014. Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two stage process. Bioresour. Technol. 155, 204-212.

Velichkova k, Sirakov I, Georgiev G. 2013. Cultivation of Scenedesmus dimorphus strain for biofuelproduction.An international j. published by Faculty of Agriculture,trakia university ,stara Zagora.Bulgaria.

Walker TL, Purton S, Becker DK, Collet C. 2005 Microalgae as bioreactors. J. Plant Cell Reports. 24: 629–641.

Xue S, Su Z, Cong W. 2011. Growth of Spirulina platensis enhanced under intermittent illumination. Journal of Biotechnology 151 (3), 271–277.

Yeesang C and Cheirsilp B. 2011. Effect of nitrogen, salt, and iron content in the growth medium and light intensity on lipid production by microalgae isolated from freshwater sources in Thailand. Bioresour. Technol. 102, 3034- 3040.

Zochleder,V, Wittenburg E, Abarzua S. 1986 Factors controlling the inhibitory effects of 3, 4_ benzo(a) pyrence on the chlorococcal alga Scenedesmus quadricauda. Algological studies/ Archive fur Hydrobiologie, Supplement Volumes 43: 281-296

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