The Effect of different levels of algae (Spirulina) on growth performance, intestinal morphology, gut microflora, carcass characteristics and some blood parameters in broiler chickens
Fatemeh Gholizadeh

Introduction: The Effect of different levels of algae (Spirulina platensis) on growth performance, intestinal morphology, gut microflora and some blood parameters in broiler chickens was investigated in this study.
Materials and methods: Experimental diets included control diet (with no additive), 3 levels of algae (1, 1.5, 2 g/kg diet), and one level of prebiotic (1 g/kg diet) as a positive control were fed to birds from 1 to 42 d of age.
Results: The results showed that adding Spirulina algae to diet increased body weight gain and decreased feed conversion ratio during starter (1-10 d), grower (11-28 d) and whole period of rearing (1- 42 d) in comparison with other groups (P<0.05). The concentration of white blood cells, IgY, IgM, Ca and P in blood serum of broilers fed with 2 g alga/ kg diet was higher than other groups, however, the concentration of malondialdehyde (MDA) was lower (P<0.05). Supplementation of diet with alga increased the number of lactobacillus in gut (P<0.05). Also, villus height and the ratio of villus height/crypt depth in duodenum, jejunum and ileum of broilers fed with 2 g alga/kg was higher than other groups (P<0.05).
Discussion and conclusion: In conclusion, Spirulina algae supplementation improved growth performance, villus height, WBC count and decreased MDA in serum of broiler chickens, so it can be considered as a useful additive in broiler chickens diet.
Spirulina, intestinal morphology, gut microflora, broiler chicken


Anbarasan V, Kumar VK, Kumar PS & Venkatachalam T. 2011. In vitro evaluation of antioxidant activity of blue green algae spirulina platensis. International Journal of Pharmaceutical Sciences and Research. 2(10): 2616-2618.

AOAC. 1990. Official method of analysis. 15th Ed. Association of Official Analytical Chemists, Washington, DC, USA.

Bellof G & Alarcon S.C. 2013. Effect of Spirulina platensis in organic broiler production Archiv. Geflug. 77: 73-80.

Bonos E, Kasapidou E, Kargopoulos A, Karampampas A, Christaki E, Florou-Paneri P & Nikolakakis I. 2016. Spirulina as a functional ingredient in broiler chicken diets. South African Journal of Animal Science. 46 (1): 94-102.

Ciftci M, Güler T, Dalkiliç B & Ertas ОN. 2005. The effect of anise oil (Pimpinella an¡ sum L.) on broiler performance. International Journal of Poultry Science. 4(11): 851-855.

Colla LM, Muccillo-Baisch AL & Costa JA. 2008. Spirulina platensis effects on the levels of total cholesterol, HDL and triacylglycerols in rabbits fed with a hypercholesterolemic diet. brazilian archives of biology and technology. 51(2): 405-411.

Farooq SM, Ebrahim AS, Subramhanya KH, Sakthivel R, Rajesh NG & Varalakshmi P. 2006. Oxalate mediated nephronal impairment and its inhibition by c-phycocyanin: A study on urolithic rats. Molecular and cellular biochemistry. 284 (1-2): 95-101.

Ferrira-Hermosillo A, Torres-Duràn PV, Shamosh-Halabe S & Juàrez-Oropeza MA. 2011. Biological effects of Spirulina and current research on its antioxidant activity. Revista Internacional de Cienciay. 2 (1): 1-13.

Fotea L, Costăchescu E, Hoha G & Leonte D. 2010. The effect of oregano essential oil (Origanum vulgare L) on broiler performance. Lucrari stiintifice. 53: 491-494.

Friedewald WT, Levy RI & Fredrickson DS. 1972. Estimation of the concentration of LDL cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry. 18:499-504.

Furbeyre H, van Milgen J, Mener T, Gloaguen M & Labussiere E. 2017. Effects of dietary supplementation with freshwater microalgae on growth performance, nutrient digestibility and gut health in weaned piglets. Animal. 11(2): 183-192.

Gognet GP, Niess E, Rodehutscord M & Pfeffer E. 2001. Algae-meal from lake Chad replacing soybean-meal in broiler diets. Archiv fur Geflugelkund. 65: 265-268.

Gordon T & Amer M. 1977. Cardiovascular disease marker, Am. J. Med. 62: 707-714.

Guban J, Korver DR, Allison GE & Tannock GW. 2006. Relationship of dietary antimicrobial drug administration with broiler performance, decreased population levels of Lactobacillus salivarius, and reduced bile salt deconjugation in the ileum of broiler chickens. Poultry science. 85: 2186-2194.

Hajati H, Hassanabadi A & Waldroup PW. 2011. Effects of dietary supplementation with pumpkin oil (Cucurbita pepo) on performance and blood fat of broiler chickens during finisher period. American Journal of Animal and Veterinary Sciences. 6 (1): 40-44.

Hemalatha K, Pugazhendy K, Jayachandran K, Jayanthi C & Meenambal M. 2012. Studies on the Protective Efficacy of Spirulina against Lead Acetate Induced Hepatotoxicity in Rattusnorvegicus. International Journal of Chemical and Analytical Science. 3: 1509-1512.

Huang ZX, Mei XT, Xu DH, Xu SB & Lv JY. 2005. Protective effects of polysacchride of Spirulina platensis and Sargassum thunbeergii on vascular of alloxan induced diabetic rats. Zhongguo Zhong yao za zhi= Zhongguo zhongyao zazhi= China journal of Chinese materia medica. 30(3), 211-215.

Jain NC. 1993. Essential of veterinary hematology. Lea and Febiger, Philadelphia. 266-277.

Kaoud AH. 2012. Effect of Spirulina platensis as a dietary supplement on broiler performance in comparison with prebiotics. Scientific Journal of Applied Research. 1: 44-48

Kaoud HA. 2015. Effect of Spirulina platensis as a dietary supplement on broiler performance in comparison with prebiotics. Speciality journal of biological science. 1(2): 1-6.

Kumar V, Tirumalai PS, Singh A, Bhatnagar AK & Shrivastavaet JN. 2013. Natural compounds from algae and Spirulina platensis & its antimicrobial activity. Indo Global journal of Pharmaceutical Science. 3 (3): 212–223.

Lokapirnasari WP, Yulianto AB & Legowo D. 2016. The Effect of Spirulina as Feed Additive to Myocardial Necrosis and Leukocyte of Chicken with Avian Influenza (H5N1) Virus Infection. Procedia Chemistry. 18: 213 – 217.

Mariey YA, Samak HR, Abou-Khashba HA, Sayed MAM & Abou-Zeid AE. 2014. Effect of using Spirulina platensis algae as a feed additives for poultry diets. Egyptian Poultry Science Journal. 34: 245-258.

Mekbungwan A, Yamauchi KE & Thongwittaya N. 2002. Intestinal morphology and enteral nutrient absorption of pigeon pea seed meal in piglets. Animal Science Journal. 73:509–16.

Nagaoka S, Shimizu K, Kaneko H, Shibayama F, Morikawa K, Kanamaru Y, Otsuka A, Hirahashi T & Kato T. 2005. A novel protein Cphycocyanin plays a crucial role in the hypocholesterolemic action of Spirulina platensis concentrate in rats. Journal of Nutrition. 135: 2425- 2430.

Pradhan J, Das BK, Sahu S, Marhual NP, Swain AK, Mishra BK & Eknath AE. 2012. Traditional antibacterial activity of freshwater microalga Spirulina platensis to aquatic pathogens. Aqua Res. 43(9):1287–1295.

Raach-Moujahed A, Hassani S, Zairi S, Bouallegue M, Darej C, Haddad B & Damergi C. 2011. Effect of dehydrated Spirulina platensis on performance and meat quality of broilers. Research Opinions in Animal and Veterinary Sciences. 1(8): 505-509.

Sallam KI. 2007. Antimicrobial and antioxidant effects of sodium acetate, sodium lactate, and sodium citrate in refrigerated sliced salmon. Food Control. 18: 566-575.

Sarada DVL, Kumar CH & Rengasamy R. 2011. Purified Cphycocyanin from Spirulina platensis (Nordstedt) geitler. A novel and potent agent against drug resistant bacteria. World Journal of Microbiology and Biotechnology. 27: 770–783.

SAS. 2001. SAS Users Guide Statistics. Version 8.2. Ed. SAS institute Inc., Cary, NC, USA.

Shanmugapriya B, Babu SS, Hariharan T, Sivaneswaran S & Anusha MB. 2015. Dietary administration of Spirulina platensis as probiotics on growth performance and histopathology in broiler chicks. International Journal of Recent Scientific Research. 6: 2650-2653.

Sivakumar J & Santhanan P. 2011. Antipathogenic activity of Spirulina platensis. Recent Research in Science and Technology. 3(4): 158–161.

Toyomizu M, Sato K, Taroda H, Kato T & Akiba Y. 2001. Effects of dietary Spirulina on meat colour in muscle of broiler chickens. British Poultry Science. 42: 197-202.

Zeweil H, Abaza IM, Zahran SM, Ahmed MH, AboulEla HM & Saad AA. 2016. Effect of Spirulina platensis as dietary supplement on some biological traits for chickens under heat stress condition. Asian Journal of Biomedical and Pharmaceutical Sciences. 6(56): 08.

Zhang HQ, Lin AP, Sun Y & Deng YM. 2001. Chemo- and radio-protective effects of polysaccharide of Spirulina platensis on hemopoietic system of mice and dogs. Acta Pharmacologica Sinica. 22(12): 1121-4.

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