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Spirulina Production in Different Sources of Nitrogen
Najmeh Gord-Noshahri, Maryam Ameri, Behrouz Jalal Ghasem

Abstract
The Cyanobacteria Spirulina is an attractive
target for its pigments, proteins, vitamins
and other high-value cell components. Also, it
can be easily and cheaply harvested by filtration
from the cultivation medium. In this study
a simple protocol was developed for Spirulina
production by using different types of nitrogen
in ammonium (Urea and (NH4)2SO4) and
nitrate (KNO3, NaNO3) forms in combination
with NPK fertilizer. Results demonstrated
high amount of nitrogen in both forms inhibited
Spirulina growth. Ammonium showed a
stronger inhibitory role than nitrate in biomass
production while increased phycocyanin content.
Best phycocyanin content occurred in
high ammonium or low nitrate concentration.
In media based on 1% Urmia lake salt and 1g/L
NPK, a combination of low concentration (0.1
-0.5g/L) of Urea and (NH4)2SO4 obtained best
results in biomass production. 1.2g/L biomass
during 14 days without any carbon source can
be compared with Zarrouk/2 medium. This
composition can be used economically for
Spirulina production since little amount of
cheap material make the possibility of Spirulina
production.
Keywords
Ammonium, Fertilizer, Nitrogen, NPK, Spirulina

References

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