The Effect of Some Environmental Factors on Biomass and Agar Content of Gracilaria corticata (Gracilariales, Rhodophyta)

The effects of some environmental parameters
on biomass and agar yield of Gracilariacorticata
were examined under laboratory
conditions. The macroalga was collected from
natural tidal pool with in Bostaneh coast (the
Persian Gulf) in May 2004. In the laboratory,
biomass and agar yield were measured after 45
days examining effect of different temperatures
(24, 27 and 32°C), photon irradiances (24, 66
and 94 photons m-2s-1), and concentrations of
ammonium phosphate [unenriched seawater
(0), 0.04 and 0.08gl-1] in culture. Except for
concentration of ammonium phosphate, other
environmental variables showed no significant
relationship (P>0.05) with biomass and agar
content of G. corticata. The agar yield derived
from the alga cultivated at 0.08gl-1 ammonium
phosphate (22.40±1.81%) was significantly
greater than the other treatments that makes G.
corticata one of the commercial agarophytes

Keywords: Biomass, Temperature, Irradiance, Gracilaria corticata, Ammonium phosphate, Agar


Bird KT, Hanisak MD, Ryther J. (1981). Chemical quali

ty and production of agar extracted from Gracilaria

tikvahiae grown in different nitrogen enrichment conditions.

Hydrobiologia. 2: 441-444.

Choi HG, Kim YS, Kim JH, Lee SJ, Park EJ, Ryu J, Nam

KW. (2006). Effects of temperature and salinity on the

growth of Gracilaria verrucosa and G. chorda, with

the potential for mariculture in Korea. Journal of Applied

Phycology.18: 269–277.

Dawes CJ, Mathieson AC, Cheney DP. (1974). Ecological

studies of Floridea neucheuma (Rhodphyta, Gigartinales)

I. Seasonal growth and reproduction. Bulletin

of Marine Science. 24: 235-273.

Deboer JA, Guigly HJ, Israel TL, D’ella CF. (1978). Nutritional

studies of two red algae. I. Growth rate as a

function of nitrogen source and concentration. Phycology

: 261-266.

Deboer JA. (1979). Effects of nitrogen enrichment on

growth rates and phycocolloid content on Gracilaria

foliifera and Neoagardhiella baileyi (Florideophyceae)

proc. International Seaweed Symposium.


D’elia CF and Deboer JA. (1978). Nutritional studies of

two red algae: II Kinetics of ammonium and nitrate. Journal of Phycology. 14: 226-272.

Destombe C, Godin J, Bodard M. (1988). The decay phase

in the life history of Gracilaria verrucosa: In: T. Stadler

J. Mollion MC. Verdus Y. Karamanos H. Morvan D.

Christiaen, (eds), The consequences in intensive cultivation.

Algal Biotechnology, Elsevier applied Science,

London, UK.

Eaton AD, Clesceri LS, Rice EW, Greenberg AE. (1998).

Standard methods for the examination of water and

waste water, American Public Health Association.

Engeldow HR, Bolton JJ. (1992). Environmental tolerance

in culture and agar content of Gracilaria verrucosa

(Hudson) Papenfuss (Rhodophyta, Gigartinales)

from Saldanha Bay. South African Journal of Botany.

(4): 263-267.

Freile-Pelegrin Y, Robledo D, Garcia-Reina G. (1995).

Seasonal agar yield in Gelidium canariensis (Grunow)

Seoane-Camba (Gelidiales, Rhodophyta) from Gran

Canaria, Spain. Journal of Applied Phycology. 7: 141–

Freile-Pelegrin Y, Robledo D, Serviere-Zaragoza E.

(1999). Gelidium robustum agar: Quality characteristics

from exploited beds and seasonality from an unexploited

bed at Southern Baja California, M´exico. Hydrobiologia.

/399: 501–507.

Freile-Pelegrin Y. (2000). Does storage time influence

yield and agar properties in the tropical agarophyte

Gracilaria cornea. Journal of Applied Phycology.

: 153–158.

Friendlander M, Shalev R, Ganor T, Strimling S. (1987).

Seasonal fluctuations of growth rate and chemical composition

of Gracilaria cf. conferta in outdoor culture in

Israel. Hydrobiologia. 151/152: 501-507.

Givernaud AM, Hassani LA, Givernaud TH, LemoineY,

Benharbet O. (1999). Biology and agar composition

of Gelidium sesquipedale harvested along

the Atlantic coast of Morocco. Hydrobiologia. 398-


Glenn E, Moore P, Akatagawa D, Himler M, Walsh A,

Nelson T. (1999). Correlation between Gracilaria parvispora

(Rhodophyta) biomass production and water

quality factors on a tropical reef in Hawaii. Aquaculture.

: 323-331.

Hanisak MD. (1990). The use of Gracilaria tikvahiae

(Gracilariales, Rhodophyta) as a model system to

understand the nitrogen nutrition of cultured seaweeds.

Hydrobiologia. 204/205: 79-87.

Israel A, Martinez-Gross M, Friendler M. (1999). Effect

of salinity and pH on growth and agar yield of Gracilaria

tenuistipitata var. liui in laboratory and outdoor

cultivation. Journal of Applied Phycology. 11: 543-

Kakita H and Kamishima H. (2006). Effects of environmental

factors and metal ions on growth of the red alga

Gracilaria chorda Holmes (Gracilariales, Rhodo

phyta). Journal of Applied Phycology. 18: 469- 474.

Lapointe BE and Ryther JH. (1978). Some aspects of

the growth and yield of Gracilaria tikvahiae in Culture.

Aquaculture. 15:185-193.

Larned S. (1998). Nitrogen versus phosphorous limited

growth and sources of nutrients for coral ceef macroalgae.

Marine Biology. 132: 409-421.

Lingell A, Ekman P, Pedersen M. (1987). Cultivation

technique for marine seaweeds allowing controlled and

optimized conditions in the laboratory and on a pilot

scale. Botanica Marina. 30: 417-424.

Marinho–Soriano E, Bourret E, Casabianca MLD, Maury

L. (1999). Agar from reproductive and vegetative

stages of Gracilaria bursa- pastoris. Bioresource Technology.

: 1-5.

Marinho–Soriano E, Silva TSF, Moreira WSC. (2001).

Seasonal variation in the biomass and agar yield from

Gracilaria Cervicoris and Hydropuntia Cornea

From Brazil. Bioresource Technology. 77: 115-120.

Marinho-Soriano E and Bourret E. (2003). Effects of

season on the yield and quality of agar from Gracilaria

species (Gracilariaceae, Rhodophyta). Bioresource

Technology. 90: 329–333.

Mercado JM, Carmona R, Niell FX. (2000). Affinity for

inorganic carbon of Gracilaria tenuistipita cultured at

low and high irradiance. Planta. 210: 758-764.

McLachlan J and Bird CJ. (1986). Gracilaria (Gigartinales,

Rhodophyta) and productivity. Aquatic Botany. 26:


Molloy FJ. (1992). Studies on the ecology and production

of seaweeds of economic and potential economic

importance on the Namibian Coast. PhD Thesis, University

of Cape Town, Cape Town South Africa.

Nelson SG. (1995). Immediate enrichment of photosynthesis

by coral reef macrophytes in response to ammonia

enrichment. Proc. 5th International Coral Reef Congress,

Tahiti. 65-70.

Nelson SG, Edward P, Conn J, Moore D, Akutagawa M.

(2001). Cultivation of Gracilaria parvispora (Rhodophyta)

in shrimp-farm effluent ditches and floating cages

in Hawaii: A two phase polyculture system. Aquaculture.

: 239-248.

Orduna-Rojas J and Robledo D. (2002). Studies on the tropical

agarophyte Gracilaria cornea J. Agaradh (Rhodophyta,

Gracilariales) from Yucatan, Mexico. II Biomass

assessment and reproductive phenology. Botanica Marina.

: 459-464.

Orduna-Rojas J, Garcia-Camacho KY, Orozco-Meyer P,

Riomsena-Rodrigez R, Pacheco-Ruiz I, Zertuche-Gonzalez

JA, Meling-Lopez AE. (2008). Agar properties of

two species of Gracilariaceae from the Gulf of California,

Mexico. Journal of Applied Phycology. 20: 169–175.

Oza RM. (1978). Studies on Indian Gracilaria. IV. Seasonal

variation in agar and gel strength of Gracilaria

corticata J. Agardh Occurring on the coast of Veraval.

Botanica Marina. 21: 165–167.

Ryther JH, Corwin N, Debusk TA, Williams LD. (1981).

Nitrogen uptake and storage by the red algae Gracilaria

tikvahiae. Aquaculture. 26: 107-115.

Singh A, Ramakrishna T, Murthy MS. (1979). Some ecological

observations on two agarophytes from India.

Hydrobiologia. 75: 185-188.

Skriptsova AV, Titlyanova TV, Titlyanova EA. (2001).

Red algae of the genus Gracilaria in the South of the

Russian Far East. Russian Journal of Marine Biology.

: 538-552.

Smit AJ, Robertson BL, Dupreez DR. (1997). Influence

of ammonium-N pulse concentrations and frequency,

tank condition and nitrogen starvation on growth rate

and biochemical composition of Gracilaria gracilis.

Journal of Applied Phycology. 8: 73-481.

Stellaroslin A. (2001a). Seasonal variation in the growth

of marine algae in relation to environmental parameters

in Arochiapuram coast. Indian Hydrobiology. 4: 86-93.

Stellaroslin A. (2001b). Seasonal variation in the agar

content of marine red algae in relation of environmental

parameters at arochiapuram coast. Indian Hydrobiology.

: 79-85.

Subbaraju DP, Ramakrishna T, Murthy MS. (1981). Effects

of some growth regulators on Gracilaria corticata

an agarophyte. Aquatic Botany. 10: 75-80.

Tsai CC, Chang JS, Sheu F, Shyu YT, Yu AYC, Wong SL,

Dai CCF, Lee TM. (2005). Seasonal growth dynamics

of Laurencia papillosa and Gracilaria coronopifolia

from a highly eutrophic reef in Southern Taiwan temperature

limitation and nutrient availability. Journal of

Experimental Marine Biology and Ecology. 315:49-69.

Wakibia JG, Anderson RJ, Keats DW. (2001). Growth

rates and agar properties of three gracilarioids in suspended

open-water cultivation in St. Helena Bay. South

African Journal of Applied Phycology. 13: 195-207.

Westermeier R, Gomez I, Rivera P. (1993). Suspended

farming of Gracilaria Chilensis (Rhodophyta, Gigartinales)

at Cariquilda River, Maullin, Chile. Aquaculture.

: 215-229.

Yang H, Zhou Y, Mao Y, Li X, LivY, Zhan F. (2005).

Growth characters and photosynthetic capacity of

Gracilaria lemaneiformis as a biofilter in a shellfish

farming area in Sanggou Bay, China. Journal of Applied

Phycology. 17: 199-206.

Yang YF, Fei XG, Song JM, Hu HY, Wang GC, Chung

IK. (2006). Growth of Gracilaria lemaneiformis under

different cultivation conditions and its effects on nutrient

removal in Chinese coastal waters. Aquaculture.

: 248-255.

Yokoya NS, Kakita H, Obika H, Kitamura T. (1999). Effects

of environmental factors and plant growth regulators

on growth of the red alga Gracilaria vermiculophylla

from Shikoka Island, Japan. Hydrobiologia.

-399: 399-374.

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